2 essential techniques of cancer cell culture kenneth g macleod and simon p langdon 1 introduction for example skov 3 ovarian cancer cell line 2 tissue culture flasks 3 complete cell culture medium for example rpmi 1640 containing 10 heat inactivated basic techniques of cell culture 21 24 cloning 1 cells 2 cell culture . Colorectal cancer is the second leading cause of cancer related death in the united states and an estimated 148300 cases will occur in 2002 representing approx 115 of all new cancers in men and. In a later targeted study 12 of 30 lung cancer cell lines were found to have different egfr mutations within the same cultures these studies indicate that the biologic mechanisms underlying heterogeneity within primary tumors may be operational in cell line models and that cell lines can be used to study heterogeneous responses to therapy. Human cancer cell lines are an integral part of drug discovery practices however modeling the complexity of cancer utilizing these cell lines on standard plastic substrata does not accurately represent the tumor microenvironment research into developing advanced tumor cell culture models in a
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